A user-friendly authoring language and development environment for graphic adventure games

This thesis discusses the implementation of a DSL and web IDE to support users with little or no programming experience with the creation of web games focused on interactive storytelling. The system has been implemented to validate the reactive programming model proposed by the evReact framework and its ability to easily represent control flow as a data structure that can be generated by a system like ours. Moreover, we have been able to persist the current state of the workflow by serializing the networks underlying the evReact model

Social life cycle assessment of an innovative industrial wastewater treatment plant

Abstract Background The social impacts generated by industrial waste treatment processes have not been studied enough, as shown in the literature. Social life cycle assessment studies have mainly focused on the assessment of products and less on industrial waste, especially wastewater, although potentially relevant from an environmental point of view, and also from a social one for various stakeholders. Purpose This case study concerns the social assessment of an innovative technology to treat the wastewater of a microelectronics company. In order to produce electronic components and semiconductors, the company has to treat and dispose of relevant wastewater streams containing various toxic substances. The wastewater streams need to be treated in order to protect the eco-system, representing a high cost for the company and a potential impact on the environment. For this reason, the company developed a LIFE project to demonstrate the viability to decrease the burdens on water bodies. The positive outcome of the test on the pilot plant paved the way for the construction of the full-scale plant that will treat all the wastewater generated by the company. The objective of this paper is the socio-economic assessment of a full-scale plant designed to treat three different kinds of wastewater. Methods The assessment of socio-economic potential impacts of a new technology has been carried out through the PSILCA (Product Social Impact Life Cycle Assessment) database implementation to evaluate 65 social indicators of a wastewater treatment plant. Results The line with the highest impact is the one which treats tetramethylammonium hydroxide; this is because this wastewater flow is the most abundant (14 and 43 times greater than the other wastewaters, respectively). The most affected stakeholder is the Local Community, followed by the Actors of the Value Chain; in fact, the results referred to the functional unit considered exceed 300,000 medium risk hours in both cases. For the Local Community this result arises from the indicator "Contribution to environmental load," which is understandable considering the object of the study since this indicator includes health effects. As far as the Value Chain Actors stakeholder is concerned, the two indicators most impacted are "Corruption" and "Social responsibility along the supply chain". The analysis conducted has also shown that upstream has a fundamental relevance for the social risks detected. Conclusions Considering the current lack of studies on both environmental and social impacts of wastewater treatment, and the fact that Social Life Cycle Assessment has not been widely used in this field, as emerged from literature review, this work is the first use of the PSILCA database to assess an industrial wastewater plant. The use of a social life cycle assessment database allows the value chain of a product system to be considered: the results show that most of the overall social risk derives from upstream sectors

Synthesis and Antiproliferative Effect of Halogenated Coumarin Derivatives

A series of 6- and 6,8-halocoumarin derivatives have been investigated as potential antiproliferative compounds against a panel of tumor and normal cell lines. Cytotoxic effects were determined by the MTT method. To investigate the potential molecular mechanism involved in the cytotoxic effect, apoptosis assay, cell cycle analysis, reactive oxygen species (ROS), and reduced glutathione analysis were performed. Among the screened compounds, coumarins 6,8-dibromo-2-oxo-2H-chromene-3-carbonitrile 2h and 6,8-diiodo-2-oxo-2H-chromene-3-carbonitrile 2k exhibited the most antiproliferative effect in thyroid cancer-derived cells TPC-1. The apoptosis assay showed that both 2h and 2k induced apoptosis in TPC-1 thyroid cancer cells. According to these experiments, both coumarins induced a slight increase in TPC-1 cells in the G2/M phase and a decrease in the S phase. A significant increase in ROS levels was observed in TPC-1 treated with diiodocoumarin 2k, while the dibromocoumarin 2h induced a decrease in ROS in a dose and time-dependent manner

Antioxidant, Antimicrobial, and Other Biological Properties of Pompia Juice

Abstract: Pompia is a Citrus species belonging to Sardinian endemic biodiversity. Health benefits were attributed to its flavedo rind extracts and essential oils while the juice qualities have never been investigated. In this paper, the antioxidant, antimicrobial, and other biological properties of Pompia juice were studied. A combined LCMS/electrochemical/biological approach was used to clarify a still debated phylogeny of this species and to explain the role of its juice phenolic compounds. A closer phylogenetic relationship with lemon and citron, rather than oranges was suggested. Sensors-based electrochemical measures, together with LCMS qualitative and quantitative analyses, revealed a high contribution of ascorbic acid and phenolics with low redox potential, isorhamnetin 3-O-rutinoside, diosmin, and diosmetin 6,8-diglucoside, to antioxidant capacity. The biological assays demonstrated a marked effect of low concentration of Pompia juice against reactive oxygen species (ROS) starting from 50 µg mL−1, and a moderate capacity to reduce ROS damages on cell membrane. Treatments with Pompia juice also resulted in a significant reduction (20%) of the metabolic activity of SW48 colon cancer cells. Lastly, MIC, MBC, and MBIC antimicrobial assays demonstrated that Pompia and lemon juices have inhibitory and antibiofilm effects against the pathogenic bacteria Pseudomonas aeruginosa, Streptococcus aureus, and Enterococcus faecali

A single synthetic small molecule that generates force against a load

Some biomolecules are able to generate directional forces by rectifying random thermal motions. This allows these molecular machines to perform mechanical tasks such as intracellular cargo transport or muscle contraction in plants and animals. Although some artificial molecular machines have been synthesized and used collectively to perform mechanical tasks, so far there have been no direct measurements of mechanical processes at the single-molecule level. Here we report measurements of the mechanical work performed by a synthetic molecule less than 5 nm long. We show that biased Brownian motion of the sub-molecular components in a hydro- gen-bonded [2]rotaxane—a molecular ring threaded onto a molecular axle—can be harnessed to generate significant directional forces. We used the cantilever of an atomic force microscope to apply a mechanical load to the ring during single-molecule pulling–relaxing cycles. The ring was pulled along the axle, away from the thermodynamically favoured binding site, and was then found to travel back to this site against an external load of 30 pN. Using fluctuation theorems, we were able to relate measurements of the work done at the level of individual rotaxane molecules to the free-energy change as previously determined from ensemble measurements. The results show that individual rotaxanes can generate directional forces of similar magnitude to those generated by natural molecular machines